File:Transcriptional-activity-around-bacterial-cell-death-reveals-molecular-biomarkers-for-cell-viability-1471-2164-9-590-S1.ogv

English: Time-lapse movie of heat-induced expression profiles. The time-lapse movie of expression profiles was constructed by the use of Windows Movie Maker version 5.1 (Microsoft Inc.). All genes were sorted in the gene order present on the B. subtilis genome and presented in 32 columns of 96 genes each. Only those genes that contained a detectable transcription level at 30°C were analyzed. This filter led to a total of 75% of the genes (3072 out of 4100 genes). The 29 frames used for the movie consist of 8 measurements from 0–12 minutes and 21 interpolations. The parameters time, temperature and CFU counts were inserted in the microarray images, which were generated with the TIGR MultiExperimentViewer version 4.0.01. The 2log ratios were colour-coded, ranging from -3 (green) to +3 (red). Note that there is one region throughout the whole series of profiles with nearly identical expression levels to the reference profile at 30°C. This region contains 67 ORFs from rplK to rpsL and encodes at least 47 different ribosomal proteins.