File:Trans-mitochondrial-coordination-of-cristae-at-regulated-membrane-junctions-ncomms7259-s2.ogv

English: Supplementary Movie 1 Animated Z-series of electron tomogram showing trans-mitochondrial cristae coordination (0:16 min). Section of heart cardiomyocyte (thickness: 197nm) in mice with naturally dysmorphic cristae (Ant1-/-;ND6P25L), imaged by dual axis electron tomography (see methods for details). The central mitochondrion makes contact with four surrounding mitochondria. At these sites of contact marking the top, left and bottom segments of the central mitochondrion, cristae form organized parallel arrays to each other. They also exhibit few fenestrations. Only at the right margin of the central mitochondrion, where it contacts non-mitochondrial cellular components (i.e., myofibrils and sarcoplasmic reticulum), are cristae disorganized. At inter-mitochondrial junctions, cristae of the central mitochondrion are aligned in space with those of neighbouring mitochondria. This can be noticed by their physical apposition throughout the three-dimensional space as the volume is visualized. Note how the near parallel transmitochondrial alignment entails cristae curvature. In so doing, cristae physically extend between two inter-mitochondrial junctions.