File:Pone.0082774.g003.tif

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English: Dinoflagellate/Viral NucleoProtein (DVNP) immunofluorescence assays (IFAs) of ethanol-fixed haemolymph samples obtained from heavily infected blue swimmer crabs Callinectes sapidus from Chesapeake Bay (USA).
DNA is shown in red and anti-DVNP is shown in green.
(A1) IFA (left) and brightfield overlay (right) of a DVNP-negative C. sapidus hyalinocyte blood cell.
(A2) IFA (left) and brightfield overlay (right) of a DVNP-negative C. sapidus granulocyte blood cell.
(A3) IFA (left) and brightfield overlay (right) of a DVNP-positive Hematodinium cell.
(B1) HematodiniumÄÄ cells are strongly labeled around the edge of their nuclei while the DNA stain concentrates in the center of the nuclei.
(B2) DVNP-labeling is absent in C. sapidus cells (red nuclei) and prominent in Hematodinium cells (green-yellow nuclei).
Date
Source New Host Range for Hematodinium in Southern Australia and Novel Tools for Sensitive Detection of Parasitic Dinoflagellates. In: PLOS ONE 8(12): e82774. doi:10.1371/journal.pone.0082774.
Author Sebastian G. Gornik, Andrea Cranenburgh, Ross F. Waller

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