File:Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv

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Lentiviral-Vectors-and-Protocols-for-Creation-of-Stable-hESC-Lines-for-Fluorescent-Tracking-and-pone.0005046.s013.ogv (Ogg Theora video file, length 7.7 s, 450 × 450 pixels, 433 kbps, file size: 404 KB)

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English: Example of cardiomyocytes labeled with αMHC-mCherry. The hESC line was engineered with αMHC-Puror_Rex-Neor and αMHC-mCherry_Rex-Blar vectors (Figure 1A) by G418 and Blasticidin selection (Methods). Phase contrast and fluorescence images show mCherry+ cardiomyocytes within the EB plated on gelatin-coated cell culture plastic without Puromycin treatment.
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Source Movie S3 from Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M (2009). "Lentiviral Vectors and Protocols for Creation of Stable hESC Lines for Fluorescent Tracking and Drug Resistance Selection of Cardiomyocytes". PLOS ONE. DOI:10.1371/journal.pone.0005046. PMID 19352491. PMC: 2662416.
Author Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot J, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch A, Price J, Conklin B, Chen H, Mercola M
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current08:08, 31 October 20127.7 s, 450 × 450 (404 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 111 kbps Completed 17:29, 1 September 2018 3.0 s
VP9 240P 50 kbps Completed 17:29, 1 September 2018 2.0 s
WebM 360P 457 kbps Completed 22:37, 14 November 2012 3.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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