File:In ovo electroporation to specifically target neural crest or placode cells.tiff
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S2 Fig. In ovo electroporation to specifically target neural crest or placode cells. (A) Representation of HH8 stage chick embryos and transverse views of microinjection into the lumen of the NT, position of the electrodes for electroporation, targeted transfection and transfected neural crest cells, but not placodal cells, after migration. (A’) Shows transfected GFP neural crest cells co-localizing with HNK1, but not Tuj1, marker. (B) Representation of HH9 stage chick embryos and transverse views of microinjection on top of the ectoderm region, position of the electrodes for electroporation, targeted transfection and transfected placodal cells, but not neural crest after migration. (B’) Shows transfected GFP placodal cells co-localizing with Tuj1, but not HNK1, marker. |
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| Source | https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002074 Bernardi YE, Sanchez-Vasquez E, Márquez RB, Piacentino ML, Urrutia H, Rossi I, et al. (2024) miR-203 secreted in extracellular vesicles mediates the communication between neural crest and placode cells required for trigeminal ganglia formation. PLoS Biol 22(7): e3002074. https://doi.org/10.1371/journal.pbio.3002074 |
| Author | Bernardi YE, Sanchez-Vasquez E, Márquez RB, Piacentino ML, Urrutia H, Rossi I, et al. |
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© 2024 Bernardi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 02:56, 29 October 2024 |  | 1,249 × 1,972 (1.65 MB) | Rasbak (talk | contribs) | {{Information |description=S2 Fig. In ovo electroporation to specifically target neural crest or placode cells. (A) Representation of HH8 stage chick embryos and transverse views of microinjection into the lumen of the NT, position of the electrodes for electroporation, targeted transfection and transfected neural crest cells, but not placodal cells, after migration. (A’) Shows transfected GFP neural crest cells co-localizing with HNK1, but not Tuj1, marker. (B) Representation of HH9 stage c... |
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