File:Application-of-a-Genetically-Encoded-Biosensor-for-Live-Cell-Imaging-of-L-Valine-Production-in-pone.0085731.s006.ogv
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[edit]DescriptionApplication-of-a-Genetically-Encoded-Biosensor-for-Live-Cell-Imaging-of-L-Valine-Production-in-pone.0085731.s006.ogv |
English: Microcolonies of the C. glutamicum ΔaceE Δpqo Δpgi sensor strain displaying phenotypic heterogeneity. Growth of six microcolonies (CGXII medium with 154 mM acetate, 222 mM glucose and 0.5% BHI) is shown in microfluidic chip devices. After a primary growth phase, cells were supplemented with 222 mM glucose and 0.5% BHI to trigger L-valine production. In the upper three colonies all cells showed a switch from growth to production. In the lower three colonies one or several single cells continued growing after initiation of the production phase. |
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Source | Video S5 from Mustafi N, Grunberger A, Mahr R, Helfrich S, Noh K, Blombach B, Kohlheyer D, Frunzke J (2014). "Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains". PLOS ONE. DOI:10.1371/journal.pone.0085731. PMID 24465669. PMC: 3895001. | ||
Author | Mustafi N, Grunberger A, Mahr R, Helfrich S, Noh K, Blombach B, Kohlheyer D, Frunzke J | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 09:51, 28 January 2014 | 32 s, 888 × 720 (1.39 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Mustafi N, Grunberger A, Mahr R, Helfrich S, Noh K, Blombach B, Kohlheyer D, Frunzke J |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Microcolonies of the C. glutamicum ?aceE ?pqo ?pgi sensor strain displaying phenotypic heterogeneity. Growth of six microcolonies (CGXII medium with 154 mM acetate, 222 mM glucose and 0.5% BHI) is shown in microfluidic chip devices. After a primary growth phase, cells were supplemented with 222 mM glucose and 0.5% BHI to trigger L-valine production. In the upper three colonies all cells showed a switch from growth to production. In the lower three colonies one or several single cells continued growing after initiation of the production phase. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |