File:V-Integrins-Are-Required-for-Mechanotransduction-in-MDCK-Epithelial-Cells-pone.0071485.s007.ogv
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Size of this JPG preview of this OGG file: 800 × 249 pixels. Other resolutions: 320 × 100 pixels | 640 × 199 pixels | 1,684 × 524 pixels.
Original file (Ogg multiplexed audio/video file, Theora/Vorbis, length 13 s, 1,684 × 524 pixels, 871 kbps overall, file size: 1.35 MB)
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[edit]DescriptionV-Integrins-Are-Required-for-Mechanotransduction-in-MDCK-Epithelial-Cells-pone.0071485.s007.ogv |
English: Timelapse imaging of GFP-Tln1 fusion protein in control, ItgαV- and Itgβ1-KD MDCK cells seeded onto collagen I substrate. Control, Itgβ1- and ItgαV-KD MDCK cells stably expressing talin-1-GFP were trypsinized, seeded onto collagen I-coated glass-bottom tissue culture dishes in serum-free cell culture medium, allowed to settle for 30–60 minutes and imaged for 4.5 hours with 3 minutes time intervals using TIRF-microscopy and a 63x oil-immersion objective. |
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Source | Movie S2 from Teravainen T, Myllymaki S, Friedrichs J, Strohmeyer N, Moyano J, Wu C, Matlin K, Muller D, Manninen A (2013). "?V-Integrins Are Required for Mechanotransduction in MDCK Epithelial Cells". PLOS ONE. DOI:10.1371/journal.pone.0071485. PMC: 3747215. | ||
Author | Teravainen T, Myllymaki S, Friedrichs J, Strohmeyer N, Moyano J, Wu C, Matlin K, Muller D, Manninen A | ||
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current | 21:10, 24 August 2013 | 13 s, 1,684 × 524 (1.35 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Teravainen T, Myllymaki S, Friedrichs J, Strohmeyer N, Moyano J, Wu C, Matlin K, Muller D, Manninen A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Timelapse imaging of GFP-Tln1 fusion protein in control, Itg?V- and Itg?1-KD MDCK cells seeded onto collagen I substrate. Control, Itg?1- and Itg?V-KD MDCK cells stably expressing talin-1-GFP were trypsinized, seeded onto collagen I-coated glass-bottom tissue culture dishes in serum-free cell culture medium, allowed to settle for 30?60 minutes and imaged for 4.5 hours with 3 minutes time intervals using TIRF-microscopy and a 63x oil-immersion objective. |
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Date and time of digitizing | 2013 |