File:Use-of-KikGR-a-photoconvertible-green-to-red-fluorescent-protein-for-cell-labeling-and-lineage-1471-213X-9-49-S1.ogv
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Use-of-KikGR-a-photoconvertible-green-to-red-fluorescent-protein-for-cell-labeling-and-lineage-1471-213X-9-49-S1.ogv (Ogg Theora video file, length 19 s, 519 × 512 pixels, 475 kbps, file size: 1.1 MB)
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[edit]DescriptionUse-of-KikGR-a-photoconvertible-green-to-red-fluorescent-protein-for-cell-labeling-and-lineage-1471-213X-9-49-S1.ogv |
English: Live imaging and photoconversion of KikGR, a green-to-red fluorescent protein, in a mouse embryo. 3D time-lapse of an E7.5 (early headfold stage - EHF) CAG::KikGR embryo from the EHF - ESom (early somite stage). KikGR is a green-to-red photoconvertible fluorescent protein. In the CAG::KikGR strain all cells express KikGR. There are three consecutive sequences in the movie. The first depicts the bright field channel alone to illustrate normal development (foregut invagination, development of the headfolds as well as somite condensation), second the red fluorescent channel (post conversion) overlayed on bright field, and third the red fluorescent channel. Two square regions of interest (ROIs) have been converted within the mesoderm. Each frame depicting fluorescence represents a 3D reconstruction of a z-stack (266 μm). The interval between slices is 7 μm. The time interval between z-stacks is 20 min. The time-lapse sequence covers 9 hours and 20 min. The embryo is suspended from its ectoplacental cone using a human eyelash. Anterior of the embryo is to the top left. |
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Date | |||
Source | Nowotschin S, Hadjantonakis A (2009). "Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos". BMC Developmental Biology. DOI:10.1186/1471-213X-9-49. PMID 19740427. PMC: 2872819. | ||
Author | Nowotschin S, Hadjantonakis A | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 00:18, 21 November 2012 | 19 s, 519 × 512 (1.1 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 1 |
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Author | Nowotschin S, Hadjantonakis A |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Live imaging and photoconversion of KikGR, a green-to-red fluorescent protein, in a mouse embryo. 3D time-lapse of an E7.5 (early headfold stage - EHF) CAG::KikGR embryo from the EHF - ESom (early somite stage). KikGR is a green-to-red photoconvertible fluorescent protein. In the CAG::KikGR strain all cells express KikGR. There are three consecutive sequences in the movie. The first depicts the bright field channel alone to illustrate normal development (foregut invagination, development of the headfolds as well as somite condensation), second the red fluorescent channel (post conversion) overlayed on bright field, and third the red fluorescent channel. Two square regions of interest (ROIs) have been converted within the mesoderm. Each frame depicting fluorescence represents a 3D reconstruction of a z-stack (266 ?m). The interval between slices is 7 ?m. The time interval between z-stacks is 20 min. The time-lapse sequence covers 9 hours and 20 min. The embryo is suspended from its ectoplacental cone using a human eyelash. Anterior of the embryo is to the top left. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |