File:Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s015.ogv
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Transportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s015.ogv (Ogg Theora video file, length 8.2 s, 512 × 512 pixels, 114 kbps, file size: 114 KB)
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[edit]DescriptionTransportation-of-Nanoscale-Cargoes-by-Myosin-Propelled-Actin-Filaments-pone.0055931.s015.ogv |
English: HMM-propelled actin filaments with quantum dots attached to the actin filaments via biotinylated CapZ at the plus-end. The actin filaments were also labelled with Alexa-488 phalloidin. The movie first illustrates a few frames recorded with the TRITC filter set to observe quantum dots. For a short sequence in the movie the filter set is changed to FITC to observe the entire filaments whereas the remaining frames were obtained using the TRITC filter set to observe the quantum dots again. Note, that only a small fraction of the filaments are labelled with quantum dots and that a large fraction of the stationary quantum dots were non-specifically attached to the surface. HMM incubation concentration 120 µg/ml. From series of experiments in Fig. 5 (A60 solution). Frame rate: 5 s−1 (real time) and image size: 85×85 µm2. |
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Source | Movie S7 from Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A (2013). "Transportation of Nanoscale Cargoes by Myosin Propelled Actin Filaments". PLOS ONE. DOI:10.1371/journal.pone.0055931. PMID 23437074. | ||
Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:42, 4 March 2013 | 8.2 s, 512 × 512 (114 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Persson M, Gullberg M, Tolf C, Lindberg A, Mansson A, Kocer A |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |