File:Transcription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s017.ogv
Transcription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s017.ogv (Ogg Theora video file, length 1 min 43 s, 464 × 344 pixels, 582 kbps, file size: 7.15 MB)
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[edit]DescriptionTranscription-Factors-MYOCD-SRF-Mesp1-and-SMARCD3-Enhance-the-Cardio-Inducing-Effect-of-GATA4-TBX5-pone.0063577.s017.ogv |
English: MEFs were initially transduced with FUW.M2rtTA and a lentivirus permitting the constitutive expression of the genetically encoded calcium indicator GCaMP3, and subsequently with the various combinations of the described transcriptional modules. Cells exhibiting flashing or transient increase in GFP intensity were detected in the group transduced with G4T5MCMDSF G4T5MCM1S3. Further analysis was performed using FIJI and an RGB lookup table was applied to visualize the range of signal intensity (Dark blue Least intense, Bright red Most intense): G4T5MCM1S3 (0∶51). |
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Source | Movie S3 from Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K (2013). "Transcription Factors MYOCD, SRF, Mesp1 and SMARCD3 Enhance the Cardio-Inducing Effect of GATA4, TBX5, and MEF2C during Direct Cellular Reprogramming". PLOS ONE. DOI:10.1371/journal.pone.0063577. PMID 23704920. PMC: 3660533. | ||
Author | Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:37, 25 May 2013 | 1 min 43 s, 464 × 344 (7.15 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Christoforou N, Chellappan M, Adler A, Kirkton R, Wu T, Addis R, Bursac N, Leong K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | MEFs were initially transduced with FUW.M2rtTA and a lentivirus permitting the constitutive expression of the genetically encoded calcium indicator GCaMP3, and subsequently with the various combinations of the described transcriptional modules. Cells exhibiting flashing or transient increase in GFP intensity were detected in the group transduced with G4T5MCMDSF G4T5MCM1S3. Further analysis was performed using FIJI and an RGB lookup table was applied to visualize the range of signal intensity (Dark blue Least intense, Bright red Most intense): G4T5MCM1S3 (0?51). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |