File:Tonic-Shock-Induces-Detachment-of--Giardia-lamblia-pntd.0000169.s001.ogv

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Tonic-Shock-Induces-Detachment-of--Giardia-lamblia-pntd.0000169.s001.ogv (Ogg Theora video file, length 41 s, 450 × 450 pixels, 146 kbps, file size: 722 KB)

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English: Detachment of fluorescently labeled Giardia from C2 BBe -1 monolayer under periodic tonicity swings of +/−150 mOsm/kg. 
          Movie plays at 50X real time. Unattached cells were flushed out with growth medium (tonicity = 320 mOsm/kg) prior to the movie. The first few frames of the movie show the remainder of this growth medium flowing through the chamber. Cells are then exposed to hypertonic medium (470 mOsm/kg) for 8 minutes (3 minutes of flow at 0.3 mL/min, then 5 minutes static exposure). Cells do not detach during this time, but they become adjusted to the higher tonicity and are primed for detachment during the next 8 minutes of exposure to hypotonic medium (150 mOsm/kg). Media alternate between hyper- and hypotonic to the end of the movie, and additional cells detach with every change.
(8.82 MB MOV)
Date
Source Video S1. from Hansen W, Fletcher D, Lohia A. "Tonic Shock Induces Detachment of Giardia lamblia". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0000169. PMID 18270543. PMC: 2238710.
Author Hansen W, Fletcher D, Lohia A
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Copyright owner: Hansen, Fletcher.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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WikiProject Open Access

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Date/TimeThumbnailDimensionsUserComment
current22:58, 30 August 201241 s, 450 × 450 (722 KB)Open Access Media Importer Bot (talk | contribs)Uploaded with the Open Access Media Importer. (test edit) botrequest

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Format Bitrate Download Status Encode time
VP9 360P 36 kbps Completed 18:46, 23 October 2018 8.0 s
VP9 240P 20 kbps Completed 18:46, 23 October 2018 5.0 s
WebM 360P 303 kbps Completed 23:27, 11 November 2012 9.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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