File:The zebrafish Pacsin3 orthologue.jpg
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Figure 3. The zebrafish Pacsin3 orthologue. (A) PACSIN family dendrogram (TreeFam accession TF313677). Mm, Mus musculus; Cf, Canis familiaris; Gg, Gallus gallus; Xt, Xenopus tropicalis; Dr Danio rerio; Tn, Tetraodon nigroviridis; Fr, Fugu rubripes; Ci, Ciona intestinalis; Ag, Anopheles gambiae; Ce, Caenorhabditis elegans. (B–H) Embryonic pacsin3 mRNA localization (purple) by whole mount in situ with pacsin3 antisense riboprobe at the various developmental stages noted. Anterior is left. Bar = 250 mm. (I) Lateral view of a 24 hpf control embryo with the notochord pseudocolored in green to highlight the location of this organ. Other structures apparent at this stage are labeled. Bar = 250 mm. (J) Indirect immunolabeling (green) of Pacsin3 with affinity-purified antibodies in the notochord at a lateral region of a fixed 24 hpf embryo, analogous to the red box in I. Nuclei are counterstained with Hoechst (blue). Bar = 50 mm. (K) Schematic of the organizational relatedness and domain structural identity/similarity between selected Syndapin/PACSIN isoform |
| Date | |
| Source | Edeling MA, Sanker S, Shima T, Umasankar PK, Ho¨ ning S, et al. (2009) Structural Requirements for PACSIN/Syndapin Operation during Zebrafish Embryonic Notochord Development. PLoS ONE 4(12): e8150. doi:10.1371/journal.pone.0008150 https://d-scholarship.pitt.edu/13299/1/Structural_Requirements.pdf |
| Author | James Keen, Thomas Jefferson University, United States of America |
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© 2009 Edeling et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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| current | 20:35, 27 April 2024 | | 714 × 995 (523 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 3. The zebrafish Pacsin3 orthologue. (A) PACSIN family dendrogram (TreeFam accession TF313677). Mm, Mus musculus; Cf, Canis familiaris; Gg, Gallus gallus; Xt, Xenopus tropicalis; Dr Danio rerio; Tn, Tetraodon nigroviridis; Fr, Fugu rubripes; Ci, Ciona intestinalis; Ag, Anopheles gambiae; Ce, Caenorhabditis elegans. (B–H) Embryonic pacsin3 mRNA localization (purple) by whole mount in situ with pacsin3 antisense riboprobe at the various developmental stages not... |
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