File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s010.ogv

English: (Related to Fig. 3B). Neutrophils rapidly engulf and kill GFP-Shigella. A 72 hpf lyz:dsRed larva was injected iv and in the adjacent mesenchyme with a sublethal inoculum of GFP-Shigella and live imaged by confocal fluorescence microscopy, every 2′34″ from 20 min pi (t = 0 on the movie) to 3 hpi (t = 2h37 on the movie). Neutrophils (red cell; yellow arrows follow two neutrophils over time in the movie) are recruited to the injected bacteria and efficiently phagocytose and kill them (diffuse GFP signal inside red cells), as soon as 20 mpi. Note a macrophage (white arrow, dsRed- phagocyte made visible by the engulfed green bacteria) that accumulated the GFP-Shigella over time (as suggested by the increase of GFP signal); by t = 1h58 it bursts and is quickly engulfed by neutrophils. At the end of the acquisition, all but few bacteria have been internalized and presumably killed by neutrophils (red cells). The maximum intensity projection (25 planes×2 µm) is shown. Scale bar, 50 µm.