File:The-Zebrafish-as-a-New-Model-for-the-In-Vivo-Study-of-Shigella-flexneri-Interaction-with-Phagocytes-ppat.1003588.s007.ogv

English: (Related to Fig. 3A). Engulfed GFP-Shigella persist in macrophages. A sublethal inoculum of GFP-Shigella was injected in the blood and adjacent mesenchyme of a 72 hpf mpeg1:G/U:mCherry larva, which was live imaged every 1′07″ from 20 mpi (t = 0 on the movie) to 2h30pi (t = 2h10 on the movie) by confocal fluorescence microscopy. At the beginning of the acquisition, the injected bacteria (green) have already adhered to 6 macrophages (red, white arrows); note that neutrophils (highlighted in green by the engulfed bacteria, #1,2 and 3, yellow arrow), having engulfed similar amounts of GFP-Shigella as the macrophages, have already started to kill the engulfed bacteria (as indicated by the diffuse green signal) in the mesenchyme near the point of injection. At the end of the acquisition, all bacteria there have been internalized and killed by neutrophils (diffuse GFP signal, yellow arrow), while those bacteria in macrophages (4 out of 6 macrophages are still there) seem intact. The maximum intensity projection (25 planes×2 µm) is shown. Scale bar, 50 µm.