File:The-Perilipin-Homologue-Lipid-Storage-Droplet-2-Regulates-Sleep-Homeostasis-and-Prevents-Learning-pbio.1000466.s006.ogv

English: Fly behavior after waking induced by starvation and sleep deprivation. Three minutes of behavior for flies after 7 h of starvation (left, n = 4), 7 h of sleep deprivation (right, n = 4), or untreated controls (middle, n = 4). cyc01 siblings were maintained in constant darkness prior to recording, which subsequently took place in the light. Flies were removed from their respective conditions, placed into Trikinetics tubes containing blue dye, allowed to acclimate for a few minutes, and were then recorded for 3 min. The video has been compressed to conserve space; however, the location of the fly in proximity to the food can be assessed. Given the transfer of flies to new tubes, the amount of sleep is relatively low. The flies depicted are a subset of flies that were used to quantify food intake for the first hour immediately after sleep deprivation and starvation. After an hour on blue dye, 13/13 flies that had been sleep deprived showed blue dye in their abdomen compared to 14/14 untreated controls. Spectrophotometric data confirmed these results (absorbance at 625 λ: 8.57×10−3/fly and 11.57×10−3/fly, respectively). Moreover, flies that were starved for 7 h and placed onto food containing blue dye displayed results similar to that seen in untreated controls (14/14 with blue dye and absorbance at 625 λ: 12.89×10−3/fly). Given the similarities in feeding behavior, it is unlikely that food intake post-treatment is responsible for ameliorating the negative effects of waking.