File:The-Kinematics-of-Cytotoxic-Lymphocytes-Influence-Their-Ability-to-Kill-Target-Cells-pone.0095248.s001.ogv

English: CD8+ T cells require direct prolonged contact with target cells to kill KC CD8+ T cells were loaded with SIINFEKL and incubated with effector CD8+ T cells from EGFP+OT-1 mice. Images were taken by confocal microscopy every 12 minutes in 2 µm Z-sections and have been shown here as maximal intensity projections. Cell permeable indicator dye in the media fluoresces red upon activation of caspase-3. Effector cells are seen to attach to KC early in the incubation period. KC death is seen at 21 hours by colour change as caspase-3 is activated, and then by blebbing. Target cell death is seen by both morphological changes of apoptosis and colour change, and appears to be accompanied by death of the effector cell also.