File:The-C-Proteins-of-Human-Parainfluenza-Virus-Type-1-Block-IFN-Signaling-by-Binding-and-Retaining-pone.0028382.s019.ogv

English: Supplemental video file for Figure 9 (M6PR and Stat2). Vero cells were infected with F170S HPIV1 for 48 h, stimulated with medium containing no FCS but IFN-β (1000 IU/ml) for 1 h and subsequently fixed, permeabilized and stained for nucleus (DAPI, blue), M6PR (green) and Stat2 (red). Images of cross-sections (z-stacks) of 0.17 µm thickness were acquired on a Leica SP5 confocal microscope and 3D reconstructions were generated using Imaris software (Bitplane, Zurich, Switzerland). For better visibility of the nuclear localization of Stat1, the first of the two rotations of the 3D reconstruction shows the Stat1 (green) and C proteins (red) channels but not the blue channel (DAPI, nucleus). The second rotation shows the blue channel (DAPI, nucleus) and the “co-localization channel” (yellow, calculated from the co-localizing signals of the red and the green channels).