File:Tbx2 directly represses Grem1 expression in the posterior hindlimb mesenchyme.png
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Figure 4. Tbx2 directly represses Grem1 expression in the posterior hindlimb mesenchyme. (A–F) Whole mount in situ hybridization analysis of Tbx2 and Grem1 expression in E12.0 and E11.5 hindlimbs of wild-type and Tbx2−/− embryos. Arrows (C, F) point to posterior expansion of Grem1 expression in Tbx2−/− limbs. White dotted lines demarcate the anterior limit of Tbx2 expression. (G–I) Comparative expression analysis of cre, driven from the mutant Tbx2-locus, and Grem1 by in situ hybridization on adjacent sagital hindlimb sections of E11.5 Tbx2cre/+ (control) and Tbx2cre/cre (mutant) embryos. Section planes are indicated by black lines (E, F). Arrowheads mark the anterior limit of cre expression (in green), and the posterior limit of Grem1 expression (in pink). False-colored overlays in (I) show posterior expansion of Grem1 in Tbx2−/− hindlimbs. (J–K) ChIP analysis using chromatin from anterior or posterior halves of E11.5 wild-type hindlimbs. Scheme (J) shows Tbx2 expression (blue) exclusively in the posterior limb half. (K) PCR detection of Tbx2-bound DNA regions. Total genomic DNA was used as positive control. In posterior limb halves Tbx2 binds to the reported Grem1 limb regulatory element but not to genomic control regions. ChIP experiments were performed in triplicates. |
| Date | |
| Source | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1003467 Farin HF, Lüdtke TH-W, Schmidt MK, Placzko S, Schuster-Gossler K, Petry M, et al. (2013) Tbx2 Terminates Shh/Fgf Signaling in the Developing Mouse Limb Bud by Direct Repression of Gremlin1. PLoS Genet 9(4): e1003467. https://doi.org/10.1371/journal.pgen.1003467 |
| Author | Farin HF, Lüdtke TH-W, Schmidt MK, Placzko S, Schuster-Gossler K, Petry M, et al. |
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© 2013 Farin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 21:14, 15 November 2024 | | 1,315 × 1,677 (2.13 MB) | Rasbak (talk | contribs) | {{Information |Description=Figure 4. Tbx2 directly represses Grem1 expression in the posterior hindlimb mesenchyme. (A–F) Whole mount in situ hybridization analysis of Tbx2 and Grem1 expression in E12.0 and E11.5 hindlimbs of wild-type and Tbx2−/− embryos. Arrows (C, F) point to posterior expansion of Grem1 expression in Tbx2−/− limbs. White dotted lines demarcate the anterior limit of Tbx2 expression. (G–I) Comparative expression analysis of cre, driven from the mutant Tbx2-locus, and Grem1... |
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