File:Survival-advantages-conferred-to-colon-cancer-cells-by-E-selectin-induced-activation-of-the-PI3K-1471-2407-11-285-S3.ogv

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Survival-advantages-conferred-to-colon-cancer-cells-by-E-selectin-induced-activation-of-the-PI3K-1471-2407-11-285-S3.ogv (Ogg Theora video file, length 1 min 24 s, 640 × 480 pixels, 1.07 Mbps, file size: 10.62 MB)

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English: An irrelevant control siRNA does not influence the adhesion of colon cancer cells to E-selectin-expressing endothelial cells in a laminar flow chamber. HUVECs were trypsinized and grown for 24 hrs on gelatin-coated slides. These endothelial cells were treated with 20 ng/ml IL-1β for 4 h to induce the expression of E-selectin. The endothelial cell cultures were then placed in a laminar flow chamber under a shear stress of 1 dyne/cm2. Live HT29 cells were transfected by electroporation with control siRNA purchased from Qiagen. Tumor cells in suspension (2 × 106 per assay) were labeled for 30 min with Calcein AM and washed twice with M199 medium before being added in the medium circulating over endothelial cells. Videos were taken directly using a camera mounted on a TE2000 fluorescence microscope at ×20 magnification to follow the adhesion of HT-29 cells to HUVECs.
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Source Porquet N, Poirier A, Houle F, Pin A, Gout S, Tremblay P, Paquet E, Klinck R, Auger F, Huot J (2011). "Survival advantages conferred to colon cancer cells by E-selectin-induced activation of the PI3K-NF?B survival axis downstream of Death receptor-3". BMC Cancer. DOI:10.1186/1471-2407-11-285. PMID 21722370. PMC: 3177907.
Author Porquet N, Poirier A, Houle F, Pin A, Gout S, Tremblay P, Paquet E, Klinck R, Auger F, Huot J
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current17:24, 17 September 20131 min 24 s, 640 × 480 (10.62 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 498 kbps Completed 01:38, 21 October 2018 1 min 9 s
VP9 360P 287 kbps Completed 01:38, 21 October 2018 1 min 13 s
VP9 240P 138 kbps Completed 01:38, 21 October 2018 47 s
WebM 360P 479 kbps Completed 17:26, 17 September 2013 1 min 26 s
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