File:Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv007.ogv
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Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv007.ogv (Ogg Theora video file, length 9.5 s, 348 × 260 pixels, 2.57 Mbps, file size: 2.91 MB)
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[edit]DescriptionSurface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv007.ogv |
English: Time-Lapse Video Corresponding to Figure 5 A Calcium responses of fura-2–pulsed AD10 CD4+ T cells were captured at 5-s intervals for 10 min. Calcium levels are displayed in pseudocolor. The speed of the video is 50 times real time. Due to the quenching effect of Mn2+ in the imaging buffer on the fluorescence of fura-2, fura-2–pulsed AD10 T cells gradually lost fluorescence intensity during the course of image capture. Note that the few T cells with continuously high calcium levels are dead cells. |
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Source | Video S7 from Ma Z, Sharp K, Janmey P, Finkel T (2008). "Surface-Anchored Monomeric Agonist pMHCs Alone Trigger TCR with High Sensitivity". PLOS Biology. DOI:10.1371/journal.pbio.0060043. PMID 18303949. PMC: 2253636. | ||
Author | Ma Z, Sharp K, Janmey P, Finkel T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:48, 16 November 2012 | 9.5 s, 348 × 260 (2.91 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Time-Lapse Video Corresponding to Figure 5 A |
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Author | Ma Z, Sharp K, Janmey P, Finkel T |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Calcium responses of fura-2?pulsed AD10 CD4+ T cells were captured at 5-s intervals for 10 min. Calcium levels are displayed in pseudocolor. The speed of the video is 50 times real time. Due to the quenching effect of Mn2+ in the imaging buffer on the fluorescence of fura-2, fura-2?pulsed AD10 T cells gradually lost fluorescence intensity during the course of image capture. Note that the few T cells with continuously high calcium levels are dead cells. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-02 |