File:Simultaneous-multiple-excitation-multiphoton-microscopy-yields-increased-imaging-sensitivity-and-1472-6750-11-20-S5.ogv
Simultaneous-multiple-excitation-multiphoton-microscopy-yields-increased-imaging-sensitivity-and-1472-6750-11-20-S5.ogv (Ogg Theora video file, length 2.7 s, 512 × 512 pixels, 3.42 Mbps, file size: 1.12 MB)
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[edit]DescriptionSimultaneous-multiple-excitation-multiphoton-microscopy-yields-increased-imaging-sensitivity-and-1472-6750-11-20-S5.ogv |
English: Time-lapse video of an acute brain slice that was imaged using SE-MPM. Time-lapse imaging of a 250-μm acute slice prepared on embryonic day 17 from a mouse embryo that underwent consecutive two-color in utero electroporation of hrGFP and DsRedII (described in the text) using SE-MPM at 970 nm. Images were collected every 10 minutes for 12 hours. Note that cross-excitation and bleed-through were problematic (yellow cells), and cell movement was compromised in this experiment. |
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Date | |||
Source | Butko M, Drobizhev M, Makarov N, Rebane A, Brinkman B, Gleeson J (2011). "Simultaneous multiple-excitation multiphoton microscopy yields increased imaging sensitivity and specificity". BMC Biotechnology. DOI:10.1186/1472-6750-11-20. PMID 21366923. PMC: 3062589. | ||
Author | Butko M, Drobizhev M, Makarov N, Rebane A, Brinkman B, Gleeson J | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:22, 20 November 2012 | 2.7 s, 512 × 512 (1.12 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 5 |
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Author | Butko M, Drobizhev M, Makarov N, Rebane A, Brinkman B, Gleeson J |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Time-lapse video of an acute brain slice that was imaged using SE-MPM. Time-lapse imaging of a 250-?m acute slice prepared on embryonic day 17 from a mouse embryo that underwent consecutive two-color in utero electroporation of hrGFP and DsRedII (described in the text) using SE-MPM at 970 nm. Images were collected every 10 minutes for 12 hours. Note that cross-excitation and bleed-through were problematic (yellow cells), and cell movement was compromised in this experiment. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |