File:Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s004.ogv
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Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s004.ogv (Ogg Theora video file, length 10 s, 600 × 300 pixels, 36 kbps, file size: 45 KB)
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[edit]DescriptionSelective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s004.ogv |
English: GFP-Salmonella growth is actively suppressed in human primary B cells. Widefield fluorescence microscopy of living cells at 37°C as in Fig. 2 C. Left panel: GFP signal, right panel: GFP signal projected on the transmission image. Images taken every 30 min. Cells were treated with Edelfosine, an apoptosis-inducing alkyl-lysophospholipid. No bacterial growth is observed in primary B cells until the cell undergoes apoptosis after 2 h, showing clear apoptosis associated morphological changes of the nucleus and membrane blebbing. After the onset of apoptosis, Salmonella expansion is observed within the apoptosing cell. Total duration: 15 hours. |
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Source | Video S3 from Souwer Y, Griekspoor A, de Wit J, Martinoli C, Zagato E, Janssen H, Jorritsma T, Bar-Ephraïm Y, Rescigno M, Neefjes J, van Ham S (2012). "Selective Infection of Antigen-Specific B Lymphocytes by Salmonella Mediates Bacterial Survival and Systemic Spreading of Infection". PLOS ONE. DOI:10.1371/journal.pone.0050667. PMID 23209805. PMC: 3510171. | ||
Author | Souwer Y, Griekspoor A, de Wit J, Martinoli C, Zagato E, Janssen H, Jorritsma T, Bar-Ephraïm Y, Rescigno M, Neefjes J, van Ham S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:40, 6 December 2012 | 10 s, 600 × 300 (45 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |