File:Sections of the tibial epiphysis of a 24-day-old rat.png
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| Description | Figure 3. Sections of the tibial epiphysis of a 24-day-old rat. (A) Confocal microscopy showing a 3D projection reconstructed from z-stack images of bone tissue labeled with calcein. Fluorescence (green) is located around the blood vessels and this gives rise to an arrangement of circles whereas the underlying growth plate cartilage is completely devoid of fluorescence. (B) Semithin section stained with toluidine blue showing a continuous monolayer of cuboidal osteoblasts (arrows) on the surface of the bone plate. Below the osteoblasts is a thick layer of blue-stained osteoid and beneath it, there is the unstained mineralized bone. A sharp boundary between osteoid and mineralized bone is observed (arrowheads). (C) Semithin section stained with toluidine blue showing the gradual transformation of the phenotype of the osteoblasts that became trapped in the osteoid (1–2) to osteoid-osteocytes (3–4) and osteocytes (5). (D) Bright field and (E) dark field images of a section hybridized with a 35S-labeled riboprobe for osteocalcin showing a continuous band of positive signal on the surface of the upper side whereas only some scattered cells are positive at the lower side. (F) TRAP-positive osteoclasts at the narrow space between the bone plate and the growth plate cartilage (red stained, arrows). (G) Higher magnification of a TRAP positive cell. (H) Image of the same cell in a semithin section stained with toluidine blue. (I) Bright field and (J) dark field images of a section hybridized with a 35S-labeled riboprobe for gelatinaseB showing that expression is restricted to the lower side. |
| Date | |
| Source | https://www.mdpi.com/1422-0067/22/2/900 Fernández-Iglesias, Á.; Fuente, R.; Gil-Peña, H.; Alonso-Durán, L.; Santos, F.; López, J.M. The Formation of the Epiphyseal Bone Plate Occurs via Combined Endochondral and Intramembranous-Like Ossification. Int. J. Mol. Sci. 2021, 22, 900. https://doi.org/10.3390/ijms22020900 |
| Author | Fernández-Iglesias, Á.; Fuente, R.; Gil-Peña, H.; Alonso-Durán, L.; Santos, F.; López, J.M. |
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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
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| current | 00:24, 21 November 2024 | | 1,555 × 2,409 (2.52 MB) | Rasbak (talk | contribs) | {{Information |Description=Figure 3. Sections of the tibial epiphysis of a 24-day-old rat. (A) Confocal microscopy showing a 3D projection reconstructed from z-stack images of bone tissue labeled with calcein. Fluorescence (green) is located around the blood vessels and this gives rise to an arrangement of circles whereas the underlying growth plate cartilage is completely devoid of fluorescence. (B) Semithin section stained with toluidine blue showing a continuous monolayer of cuboidal osteo... |
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