File:Sections of the tibial epiphysis of 19 day-old rats.png

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Description Figure 2. Sections of the tibial epiphysis of 19 day-old rats. (A) Section stained with von Kossa and counterstained with toluidine blue showing that the epiphyseal bony tissue displays a “bicycle wheel” pattern at the upper portion and a bridge-like disposition at the lower portion. (B) Semithin section stained with toluidine blue showing rounded hollows (arrowheads) and ridges (arrows) in the epiphyseal cartilage. Boxed area is shown at a higher magnification in figure G. (C) Section stained with von Kossa and counterstained with toluidine blue showing that the cell composition differs between the upper and lower surfaces of the mineralized plate-like elements. (D) Section stained with von Kossa and counterstained with toluidine blue showing a moderately dilated capillary interposed between the cartilage and the bone forming cells. It is close to the surface of the cartilage, where a thin band of less stained cartilaginous matrix is present (arrows). Boxed area is shown at a higher magnification in figure E. (E) Magnification of the boxed area in D showing the close contact between the capillary and the cartilage. (F) Immunohistochemistry for CD31 showing positive capillaries at the metaphyseal chondro–osseous junction (arrows) whereas virtually no signal is visible in capillaries located at the epiphyseal end (arrowheads). (G) Magnification of the boxed area in B showing that the cartilage is directly covered by a layer of mineralized tissue that is weakly stained. Note that chondrocytes are not hypertrophic and have a well-preserved structure. Cells at the surface are flattened (arrowheads) and change to cuboidal at mid-depth of the hollow (arrow) with a transition zone in between (asterisks). (H) Section of a region equivalent to that shown in figure G stained with Alcian blue/acid fuchsin. The two tissues are stained differentially, with a sharp boundary visible between them. (I) Equivalent region to those shown in figures G and H stained von Kossa and counterstained with toluidine blue showing mineralized matrix being directly deposited on the cartilage.
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Source https://www.mdpi.com/1422-0067/22/2/900 Fernández-Iglesias, Á.; Fuente, R.; Gil-Peña, H.; Alonso-Durán, L.; Santos, F.; López, J.M. The Formation of the Epiphyseal Bone Plate Occurs via Combined Endochondral and Intramembranous-Like Ossification. Int. J. Mol. Sci. 2021, 22, 900. https://doi.org/10.3390/ijms22020900
Author Fernández-Iglesias, Á.; Fuente, R.; Gil-Peña, H.; Alonso-Durán, L.; Santos, F.; López, J.M.
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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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current00:39, 21 November 2024Thumbnail for version as of 00:39, 21 November 20241,998 × 2,969 (3.22 MB)Rasbak (talk | contribs){{Information |Description=Figure 2. Sections of the tibial epiphysis of 19 day-old rats. (A) Section stained with von Kossa and counterstained with toluidine blue showing that the epiphyseal bony tissue displays a “bicycle wheel” pattern at the upper portion and a bridge-like disposition at the lower portion. (B) Semithin section stained with toluidine blue showing rounded hollows (arrowheads) and ridges (arrows) in the epiphyseal cartilage. Boxed area is shown at a higher magnification in f...

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