File:Repulsive-cues-combined-with-physical-barriers-and-cell–cell-adhesion-determine-progenitor-cell-ncomms11288-s7.ogv

English: Supplementary Movie 6 High magnification view of a germ cell expressing Lifeact-Ruby protein labeling its actin structures at the cell front (presented in green) and farnesylated EGFP labeling the Golgi apparatus at the cell back (presented in red), as it interacts with the developing gut (labeled by a sox17:egfp transgene, presented in red). A SiMView light-sheet microscope was used to image a 25hpf embryo. Actin structures form at the side and the back of the PGC following its contact with the physical barrier and migration away from it. White arrows indicate the polarity of the cell. Scale bar 5μm. Dorsal view, anterior up. See also Fig. 6c.