File:Redistribution-of-Actin-during-Assembly-and-Reassembly-of-the-Contractile-Ring-in-Grasshopper-pone.0004892.s011.ogv
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Redistribution-of-Actin-during-Assembly-and-Reassembly-of-the-Contractile-Ring-in-Grasshopper-pone.0004892.s011.ogv (Ogg Theora video file, length 3.4 s, 256 × 256 pixels, 111 kbps, file size: 46 KB)
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[edit]DescriptionRedistribution-of-Actin-during-Assembly-and-Reassembly-of-the-Contractile-Ring-in-Grasshopper-pone.0004892.s011.ogv |
English: Cortical flow of actin filaments in grasshopper spermatocytes. Video S11 corresponds to Figure 8A. Time-lapse sequences of fluorescence images, captured every 30 seconds, showed cortical flow of actin filaments labeled with Alexa 488 phalloidin. Flow of actin was induced by collapsing the spindle (labeled “sp” in Figure 8A) and repositioning it near the cortex. Note that the signal cleared over time in the region closest to the spindle, as the actin flowed to the cortex on the opposite side of the cell. The displaced, autofluorescent mitochondria were seen as large white patches within the cell (labeled “m” in Fig 8A). The mitochondria were not part of the cortical flow; rather their displacement was presumably due to the elongation of dynamic spindle microtubules, with which they were associated. |
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Source | Video S11 from Alsop G, Chen W, Foss M, Tseng K, Zhang D (2009). "Redistribution of Actin during Assembly and Reassembly of the Contractile Ring in Grasshopper Spermatocytes". PLOS ONE. DOI:10.1371/journal.pone.0004892. PMID 19287500. PMC: 2654139. | ||
Author | Alsop G, Chen W, Foss M, Tseng K, Zhang D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:37, 21 November 2012 | 3.4 s, 256 × 256 (46 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Alsop G, Chen W, Foss M, Tseng K, Zhang D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Cortical flow of actin filaments in grasshopper spermatocytes. Video S11 corresponds to Figure 8A. Time-lapse sequences of fluorescence images, captured every 30 seconds, showed cortical flow of actin filaments labeled with Alexa 488 phalloidin. Flow of actin was induced by collapsing the spindle (labeled ?sp? in Figure 8A) and repositioning it near the cortex. Note that the signal cleared over time in the region closest to the spindle, as the actin flowed to the cortex on the opposite side of the cell. The displaced, autofluorescent mitochondria were seen as large white patches within the cell (labeled ?m? in Fig 8A). The mitochondria were not part of the cortical flow; rather their displacement was presumably due to the elongation of dynamic spindle microtubules, with which they were associated. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |