File:Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s010.ogv

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Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s010.ogv (Ogg Theora video file, length 10 s, 508 × 508 pixels, 604 kbps, file size: 747 KB)

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English: Tubulin labeling confirms whole spindle motion in CENP-W depleted cells. HeLa-H2B-GFP cells were transfected with mCherry-tubulin to directly visualize spindle motion following transfection with pooled siRNAs against CENP-W. It is clear that the entire spindle is moving within cells that exhibit rolling. The spindle poles can be seen to split in some of these cells, as though the forces associated with spindle movement are able to disrupt spindle pole integrity. This may account for the multipolar spindles frequently observed in fixed specimens of CENP-W siRNA-treated cells.
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Source Video S4 from Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K (2011). "Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State". PLOS Biology. DOI:10.1371/journal.pbio.1001082. PMID 21695110. PMC: 3114758.
Author Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K
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Date/TimeThumbnailDimensionsUserComment
current22:26, 14 November 201210 s, 508 × 508 (747 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 241 kbps Completed 09:16, 14 October 2018 6.0 s
VP9 360P 137 kbps Completed 09:16, 14 October 2018 5.0 s
VP9 240P 83 kbps Completed 09:16, 14 October 2018 4.0 s
WebM 360P 515 kbps Completed 16:21, 15 November 2012 7.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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