File:Predominant-membrane-localization-is-an-essential-feature-of-the-bacterial-signal-recognition-1741-7007-7-76-S2.ogv
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Predominant-membrane-localization-is-an-essential-feature-of-the-bacterial-signal-recognition-1741-7007-7-76-S2.ogv (Ogg Theora video file, length 10 s, 295 × 188 pixels, 58 kbps, file size: 71 KB)
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[edit]DescriptionPredominant-membrane-localization-is-an-essential-feature-of-the-bacterial-signal-recognition-1741-7007-7-76-S2.ogv |
English: Movie S2 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(B3) mutant derivative. The GFP-tagged FtsY(B3) mutant derivative was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(B3) derivative exhibits reduced activity due to a triple-point mutation within the first lipid-binding domain of FtsY. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player. |
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Source | Mircheva M, Boy D, Weiche B, Hucke F, Graumann P, Koch H (2009). "Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor". BMC Biology. DOI:10.1186/1741-7007-7-76. PMID 19912622. PMC: 2780400. | ||
Author | Mircheva M, Boy D, Weiche B, Hucke F, Graumann P, Koch H | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 10:12, 20 November 2012 | 10 s, 295 × 188 (71 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Mircheva M, Boy D, Weiche B, Hucke F, Graumann P, Koch H |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie S2 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(B3) mutant derivative. The GFP-tagged FtsY(B3) mutant derivative was expressed from a plasmid in DH5? and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(B3) derivative exhibits reduced activity due to a triple-point mutation within the first lipid-binding domain of FtsY. Z stacks were acquired with 0.1 ?m spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |