File:Pituitary Specification of Anterior Cranial Placode-Derived Human Pluripotent Stem Cells (hPSCs).jpg

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Description Pituitary Specification of Anterior Cranial Placode-Derived hPSCs (A) Schematic representation of pituitary gland in vivo development and protocol for directed differentiation of hPSCs into anterior pituitary-like cells. ICM/Epibl., inner cell mass/epiblast; NNE, non-neural ectoderm. (B) Real-time PCR analysis comparing expression of key genes involved in pituitary development in LSB, pituitary condition, and medium conditioned by hypothalamic neuroectoderm (Hypothalamus CM) after 15 days of differentiation in the respective medium. Values are normalized to GAPDH and gene expression on day 15 of lens differentiation (E6 only) and plotted as mean ± SEM of at least four independent experiments. *p < 0.05, ***p < 0.001 compared with E6 only condition on day 15. (C) Immunofluorescence analysis comparing expression of PITX1 and LHX3 after 15 days of differentiation under lens or pituitary conditions as well as expression of HESX1 and SIX3/6 on day 15 of pituitary differentiation. Scale bars, 50 mm. (D) Immunofluorescence comparison of cells differentiated for 15 days under either pituitary or hypothalamus condition. Cells were stained for either FOXG1 (Pituitary) or NKX2.1 (Hypothalamus). Scale bars, 50 mm. (E) qRT-PCR analysis of day 0-15 cells differentiated under pituitary or hypothalamic ectoderm condition probing for NKX2.1 and FOXG1. Values have been normalized to GAPDH and expression in day-6 placode cells and are plotted as means ± SEM of two to four independent experiments. See also Figures S2 and S3.
Date 2016-06-14-
Source https://www.researchgate.net/publication/303982389_Derivation_of_Diverse_Hormone-Releasing_Pituitary_Cells_from_Human_Pluripotent_Stem_Cells Derivation of Diverse Hormone-Releasing Pituitary Cells from Human Pluripotent Stem Cells. Stem Cell Reports Vol. 6 858–872 June 14, 2016 DOI:10.1016/j.stemcr.2016.05.005
Author Bastian Zimmer, Jinghua Piao, Kiran Ramnarine, Mark J. Tomishima, Viviane Tabar, Lorenz Studer
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current21:10, 30 October 2024Thumbnail for version as of 21:10, 30 October 2024850 × 906 (176 KB)Rasbak (talk | contribs){{Information |description=Pituitary Specification of Anterior Cranial Placode-Derived hPSCs (A) Schematic representation of pituitary gland in vivo development and protocol for directed differentiation of hPSCs into anterior pituitary-like cells. ICM/Epibl., inner cell mass/epiblast; NNE, non-neural ectoderm. (B) Real-time PCR analysis comparing expression of key genes involved in pituitary development in LSB, pituitary condition, and medium conditioned by hypothalamic neuroectoderm (Hypotha...

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