File:Overcoming-Multidrug-Resistance-via-Photodestruction-of-ABCG2-Rich-Extracellular-Vesicles-pone.0035487.s003.ogv

English: Imaging of photodestruction of EVs and gradual nuclear accumulation of C-1371 upon illumination of MCF-7/MR cells. MCF-7/MR cells were grown as described in Figure 2 legend and exposed to C-1371 (10 µM) for 24 h at 37°C to allow for its accumulation within EVs. Then, random fields were continuously exposed to blue light using the GFP+DsRED longpass filter and photographed each 5 sec for 120 cycles (a total of 10 min) using the ted Cell-Observer microscope at a ×630 magnification. The obtained time-lapse images were animated into a video using LSM image browser software (Zeiss Inc.).