File:Neisseria-meningitidis-Differentially-Controls-Host-Cell-Motility-through-PilC1-and-PilC2-pone.0006834.s003.ogv
Neisseria-meningitidis-Differentially-Controls-Host-Cell-Motility-through-PilC1-and-PilC2-pone.0006834.s003.ogv (Ogg Theora video file, length 22 s, 300 × 225 pixels, 974 kbps, file size: 2.5 MB)
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[edit]DescriptionNeisseria-meningitidis-Differentially-Controls-Host-Cell-Motility-through-PilC1-and-PilC2-pone.0006834.s003.ogv |
English: Live-imaging of ME180 cells infected with strain NmPilC1ind. ME180 cells were infected with meningococcal strains NmPilC1ind (PilC1ind/PilC2-) that expresses pilC1 under the control of an IPTG-inducible promoter. Cells were monitored continuously before infection (in the absence of IPTG), after addition of infecting bacteria (MOI 50, no IPTG), and after the induction of PilC1 expression by addition of IPTG to the medium. Adhesion to ME180 cells relies on the expression of PilC1, upon IPTG-mediated induction. However, cellular motility is reduced in the case of PilC1-mediated infection, whereas it remains unaffected in the case of PilC2-mediated adhesion (Video S2). |
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Source | Video S1 from Morand P, Drab M, Rajalingam K, Nassif X, Meyer T (2009). "Neisseria meningitidis Differentially Controls Host Cell Motility through PilC1 and PilC2 Components of Type IV Pili". PLOS ONE. DOI:10.1371/journal.pone.0006834. PMID 19718432. PMC: 2729722. | ||
Author | Morand P, Drab M, Rajalingam K, Nassif X, Meyer T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 21:58, 30 October 2012 | 22 s, 300 × 225 (2.5 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Morand P, Drab M, Rajalingam K, Nassif X, Meyer T |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live-imaging of ME180 cells infected with strain NmPilC1ind. ME180 cells were infected with meningococcal strains NmPilC1ind (PilC1ind/PilC2-) that expresses pilC1 under the control of an IPTG-inducible promoter. Cells were monitored continuously before infection (in the absence of IPTG), after addition of infecting bacteria (MOI 50, no IPTG), and after the induction of PilC1 expression by addition of IPTG to the medium. Adhesion to ME180 cells relies on the expression of PilC1, upon IPTG-mediated induction. However, cellular motility is reduced in the case of PilC1-mediated infection, whereas it remains unaffected in the case of PilC2-mediated adhesion (Video S2). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |
Structured data
2009
application/ogg
- Videos of cell signaling
- Videos of applied microbiology
- Videos of medical microbiology
- Videos of diseases and disorders due to bacteria
- Videos of infections of central nervous system
- Bacterial adhesion
- Base sequence
- Western blot
- Cell lines
- Cell movement
- DNA primers
- Fimbriae proteins
- Bacterial fimbriae
- Fluorescent antibody technique
- HeLa cells
- Neisseria meningitidis
- Epidermal growth factor receptor
- Media from PLOS ONE