File:Monitoring-G-protein-coupled-receptor-and-β-arrestin-trafficking-in-live-cells-using-enhanced-ncomms12178-s4.ogv

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Original file ‎(Ogg Theora video file, length 6.0 s, 1,024 × 512 pixels, 5.7 Mbps, file size: 4.08 MB)

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English: Supplementary Movie 1 HEK293 cells co-expressing rGFP-CAAX and βarr2-RlucII were stimulated or not with 100 nM AngII. The change of the cells morphology (e.g. shrinking) reveals its response to the agonist. Luminescence was captured as indicated in the materials and methods section following prolume purple coelenterazine using the BRET donor filter (370 nm - 450 nm) for the left panel and the and BRET acceptor filter (480LP) for the right panel. The video was generated by capturing images for 20 s every 30 s for a period of 20 min.
Date
Source Video file from Namkung Y, Le Gouill C, Lukashova V, Kobayashi H, Hogue M, Khoury E, Song M, Bouvier M, Laporte S (2016). "Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET". Nature Communications. DOI:10.1038/ncomms12178. PMID 27397672. PMC: 4942582.
Author Namkung Y, Le Gouill C, Lukashova V, Kobayashi H, Hogue M, Khoury E, Song M, Bouvier M, Laporte S
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current02:32, 27 October 20166.0 s, 1,024 × 512 (4.08 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 1.08 Mbps Completed 17:42, 3 September 2018 8.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 341 kbps Completed 17:42, 3 September 2018 5.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 114 kbps Completed 17:42, 3 September 2018 3.0 s
Streaming 240p (VP9) 115 kbps Completed 08:04, 3 February 2024 1.0 s
WebM 360P 510 kbps Completed 02:32, 27 October 2016 5.0 s
Streaming 144p (MJPEG) 234 kbps Completed 12:51, 13 November 2023 1.0 s

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