File:Mitotic spindle in Arabidopsis primary root meristem cells (anaphase).tif

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Mitotic_spindle_in_Arabidopsis_primary_root_meristem_cells_(anaphase).tif (761 × 254 pixels, file size: 212 KB, MIME type: image/tiff)

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English: Сonfocal image of mitotic microtubules (mitotic spindle; green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in root meristem cells 4-day old Arabidopsis thaliana (Col-0) visualized by whole-mount tubulin immunolabeling (Komis and Šamaj, 2014). Image was documented with a Zeiss 710 confocal laser scanning microsope (CLSM) platform (Carl Zeiss, Germany) at the Centre of the Region Haná for Biotechnological and Agricultural Research, (Olomouc, Czech Republic) by the research fellow of the "Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine" (Kyiv, Ukraine). Laser line used for GFP exitation – Ar 488 nm, for DAPI – 405 nm. The sum image represents maximum intensity projection of Z-stacks series reconstructed in Zeiss Zen 2012 software. Scale bar: 5 um.
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Author Yuliya Krasylenko

Конфокальна мікрофотографія мітотичних мікротрубочок (мітотичне веретено; зелений сигнал від анти-тубуліну, кон’югованого з барвником Alexa Fluor 488) та ядра (ДНК; блакитний сигнал від DAPI) у клітинах меристеми головних коренів чотириденних проростків Arabidopsis thaliana (Col-0), візуалізованих за допомогою техніки whole-mount tubulin immunolabeling (Komis and Šamaj, 2014). Зображення отримано за допомогою лазерного скануючого конфокального мікроскопа Zeiss 710 (Carl Zeiss, Germany) у Центрі біотехнологічних та сільськогосподарських досліджень регіону Хана (м. Оломоуц, Чеська Республіка) науковим співробітником ”Інститут харчової біотехнології та геноміки НАН України” (м. Київ, Україна). Для збудження флюорофору GFP використано аргоновий лазер з довжиною хвилі 488 нм. Сумарне зображення являє собою накладання серії оптичних зрізів (так званих Z-стеків) у програмному забезпеченні Zeiss Zen 2012. Масштаб мікрофотографії: 5 мкм.

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current23:57, 28 October 2016Thumbnail for version as of 23:57, 28 October 2016761 × 254 (212 KB)Yuliya Krasylenko (talk | contribs)User created page with UploadWizard

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