File:Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s003.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
Mitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s003.ogv (Ogg Theora video file, length 4.0 s, 520 × 512 pixels, 355 kbps, file size: 173 KB)
File information
Structured data
Captions
Summary
[edit]DescriptionMitochondrial-and-Plasma-Membrane-Pools-of-Stomatin-Like-Protein-2-Coalesce-at-the-Immunological-pone.0037144.s003.ogv |
English: Stability of SLP-2 clusters during T cell activation. Jurkat T cells stably expressing SLP-2-gfp were incubated on polylysine-coated glass bottom microwell dishes for 10 minutes to allow cells to adhere. Adherent Jurkat T cells were then stimulated with OKT3 (1 µg/ml) for 15 minutes and then placed under confocal microscope. Forty images were acquired over a 375 seconds, with three images acquired before photobleaching. Area of cell indicated by arrow was photobleached using 100% of laser power for 3 seconds and the movement of SLP-2-gfp back into photobleached area was measured. Note that, in the stimulated T cell, it corresponds to an area with SLP-2-gfp clusters. Stimulated Jurkat T cells show little to no recovery of gfp signal in photobleached area within the examined time window. |
||
Date | |||
Source | Video S3 from Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J (2012). "Mitochondrial and Plasma Membrane Pools of Stomatin-Like Protein 2 Coalesce at the Immunological Synapse during T Cell Activation". PLOS ONE. DOI:10.1371/journal.pone.0037144. PMID 22623988. PMC: 3356372. | ||
Author | Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 21:20, 30 October 2012 | 4.0 s, 520 × 512 (173 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Christie D, Kirchhof M, Vardhana S, Dustin M, Madrenas J |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |