File:Microtubules-Depolymerization-Caused-by-the-CK1-Inhibitor-IC261-May-Be-Not-Mediated-by-CK1-Blockage-pone.0100090.s009.ogv

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Microtubules-Depolymerization-Caused-by-the-CK1-Inhibitor-IC261-May-Be-Not-Mediated-by-CK1-Blockage-pone.0100090.s009.ogv (Ogg Theora video file, length 24 s, 302 × 227 pixels, 3.35 Mbps, file size: 9.64 MB)

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English: Microtubule depolymerization by IC261 treatment is reversible. CV-1 cells expressing EYFP-tubulin were treated at time point “0 min” with 3.2 µM IC261 and observed by time-resolved fluorescence microscopy. The spindle apparatus of the exemplary cell shown here was dissolved within 8 min. At time point “10 min” IC261 was removed by exchange of media. Within a few minutes spindle MTs were built up again (“15 min”) and 20 min after removal a morphologically unimpaired spindle apparatus had been developed (“30 min”). After 2 h the cell proceeded into anaphase and cytokinesis (“155 min”).
Date
Source Movie S5 from Stoter M, Kruger M, Banting G, Henne-Bruns D, Knippschild U (2014). "Microtubules Depolymerization Caused by the CK1 Inhibitor IC261 May Be Not Mediated by CK1 Blockage". PLOS ONE. DOI:10.1371/journal.pone.0100090. PMID 24937750. PMC: 4061085.
Author Stoter M, Kruger M, Banting G, Henne-Bruns D, Knippschild U
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Date/TimeThumbnailDimensionsUserComment
current21:57, 11 July 201424 s, 302 × 227 (9.64 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 248 kbps Completed 05:46, 7 November 2024 34 s
WebM 360P 440 kbps Completed 00:00, 2 December 2023 3.0 s
QuickTime 144p (MJPEG) 837 kbps Completed 05:46, 7 November 2024 1.0 s

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