File:Mapping-molecules-in-scanning-far-field-fluorescence-nanoscopy-ncomms8977-s2.ogv
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[edit]DescriptionMapping-molecules-in-scanning-far-field-fluorescence-nanoscopy-ncomms8977-s2.ogv |
English: Supplementary Movie 1 A 3D molecular map of the TfR (stained with aptamer in HEK293 cells) is reconstructed from the photon statistics of confocal and STED measurements. Isosurfaces of the molecular map which embrace 70% of the overall number of molecules in the corresponding cluster are plotted in the animation. The cluster segmentation is performed with the built in watershed function in MATLAB. 0 - 5 s: reconstruction from the photon statistics of confocal recording (Red). 6 - 11 s: reconstruction from the photon statistics of confocal and STED recording (Cyan). 19 - 31 s: reconstruction from the photon statistics of confocal and STED recording with color code representing the number of molecules in each cluster. The full region is 8 x 8 x 2 μm. |
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Source | Video file from Ta H, Keller J, Haltmeier M, Saka S, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell S (2015). "Mapping molecules in scanning far-field fluorescence nanoscopy". Nature Communications. DOI:10.1038/ncomms8977. PMID 26269133. PMC: 4557268. | ||
Author | Ta H, Keller J, Haltmeier M, Saka S, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell S | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 09:31, 31 October 2016 | 36 s, 1,600 × 840 (8.33 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 1 |
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Author | Ta H, Keller J, Haltmeier M, Saka S, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | A 3D molecular map of the TfR (stained with aptamer in HEK293 cells) is reconstructed from the photon statistics of confocal and STED measurements. Isosurfaces of the molecular map which embrace 70% of the overall number of molecules in the corresponding cluster are plotted in the animation. The cluster segmentation is performed with the built in watershed function in MATLAB. 0 - 5 s: reconstruction from the photon statistics of confocal recording (Red). 6 - 11 s: reconstruction from the photon statistics of confocal and STED recording (Cyan). 19 - 31 s: reconstruction from the photon statistics of confocal and STED recording with color code representing the number of molecules in each cluster. The full region is 8 x 8 x 2 μm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-08-13 |