File:LDs are distributed cortically in the zebrafish blastodisc.jpg
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| Description | Figure 1. Lipid droplets (LDs) are distributed cortically in the zebrafish blastodisc.: (a) DIC image of zebrafish blastodisc at 4 cell stage (animal-pole view). Arrowheads point to the LDs. (b) Nile Red labeled 3D-rendered confocal image of blastodisc showing cortical distribution of LDs. (c) Nile Red stained images of LD (upper panel) and artificially synthesized giant vesicle (lower panel). (d) (i) DIC (ii) fluorescent (iii) merge images, of Nile Red stained de-yolked blastodisc showing co-localization of Nile Red signal (ii) with the granules of (i) conforming LD identity. (e) Mean LDD versus time averaged over three embryos, error bars are standard error of mean (SEM). (f) Time lapse images of blastodisc (lateral orientation) showing migration of LDs to cortical regions of the embryo. The white lines denote the trajectories of 5 representative LDs. ‘A’ and ‘V’ denote the animal and vegetal pole of the embryo. Scale bar 25 μm in (a) and (f), 8 μm in (c), 150 μm in (b) and 100 μm in (d). |
| Date | |
| Source |
https://www.researchgate.net/publication/281676795_Turnover_of_the_actomyosin_complex_in_zebrafish_embryos_directs_geometric_remodelling_and_the_recruitment_of_lipid_droplets Turnover of the actomyosin complex in zebrafish embryos directs geometric remodelling and the recruitment of lipid droplets. Sci. Rep. 5, 13915; doi: 10.1038/srep13915 (2015). |
| Author | Dutta, A. and Kumar Sinha, D. |
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| current | 20:44, 6 May 2024 | | 1,451 × 1,024 (747 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 1. Lipid droplets (LDs) are distributed cortically in the zebrafish blastodisc.: (a) DIC image of zebrafish blastodisc at 4 cell stage (animal-pole view). Arrowheads point to the LDs. (b) Nile Red labeled 3D-rendered confocal image of blastodisc showing cortical distribution of LDs. (c) Nile Red stained images of LD (upper panel) and artificially synthesized giant vesicle (lower panel). (d) (i) DIC (ii) fluorescent (iii) merge images, of Nile Red stained de-y... |
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