File:Insulin-and-insulin-like-growth-factor-II-endocytosis-and-signaling-via-insulin-receptor-B-1478-811X-11-18-S5.ogv
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Insulin-and-insulin-like-growth-factor-II-endocytosis-and-signaling-via-insulin-receptor-B-1478-811X-11-18-S5.ogv (Ogg Theora video file, length 51 s, 254 × 254 pixels, 448 kbps, file size: 2.72 MB)
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[edit]DescriptionInsulin-and-insulin-like-growth-factor-II-endocytosis-and-signaling-via-insulin-receptor-B-1478-811X-11-18-S5.ogv |
English: Additional file 5: Movie S1 Visualization of IGF-II-biot-QD655 bound to IR-B-GFP and endocytosed. HeLa cells expressing IR-B-GFP were labeled in vivo with 50 nM IGF-II-biot and 2 nM QD655, incubated at 37°C for 1 hour, treated for 5 min with 0.1 mM Na-glycine pH 3.0, NaCl 0.5 M and fixed in 3.7% PFA. Images were taken with a confocal microscope by acquisition of 64 z-slices with 16 μm step size. Deconvolution was performed with Huygens Scientific Volume Imaging Huygens Professional Version 3.6, applying the Quick maximum likelihood estimation algorithm. The movie shows tridimensional reconstruction using the surface renderer process observed from different angles. |
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Source | Giudice J, Barcos L, Guaimas F, Penas-Steinhardt A, Giordano L, Jares-Erijman E, Coluccio Leskow F (2013). "Insulin and insulin like growth factor II endocytosis and signaling via insulin receptor B". Cell Communication and Signaling. DOI:10.1186/1478-811X-11-18. PMID 23497114. PMC: 3607927. | ||
Author | Giudice J, Barcos L, Guaimas F, Penas-Steinhardt A, Giordano L, Jares-Erijman E, Coluccio Leskow F | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:37, 28 March 2013 | 51 s, 254 × 254 (2.72 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 5: Movie S1 |
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Author | Giudice J, Barcos L, Guaimas F, Penas-Steinhardt A, Giordano L, Jares-Erijman E, Coluccio Leskow F |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |