File:Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S4.ogv

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Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S4.ogv (Ogg Theora video file, length 3.3 s, 502 × 226 pixels, 476 kbps, file size: 192 KB)

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English: Additional File 4 Nuclear contraction during wound induced cell death The hypocotyl of an agarose imbedded nuclear marker line (N6) was wounded with a razor blade and them imaged at 2 minute intervals by collecting confocal Z-series. Propidium iodide was included in the imaging buffer to monitor plasma membrane integrity. Following wounding,, the nucleus on the left displays hypertrophy relative to the normal lentoid shape of unwounded cells (see the nuclei in cells on the right hand side in the movie). Over the course of the time series, the nucleus contracts concomitant with a decrease in GFP fluorescence, most evident in the time series between the 16 and 20 minute time points shown. This is followed later by intense staining of the contracted nucleus by propidium iodide.
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Source Cutler S, Somerville C (2005). "Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide induced cell death". BMC Plant Biology. DOI:10.1186/1471-2229-5-4. PMID 15796778. PMC: 1087855.
Author Cutler S, Somerville C
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Date/TimeThumbnailDimensionsUserComment
current19:48, 5 December 20123.3 s, 502 × 226 (192 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 140 kbps Completed 13:24, 29 August 2018 2.0 s
WebM 360P 259 kbps Completed 14:40, 30 November 2023 1.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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