File:Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S4.ogv
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Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S4.ogv (Ogg Theora video file, length 3.3 s, 502 × 226 pixels, 476 kbps, file size: 192 KB)
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[edit]DescriptionImaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S4.ogv |
English: Additional File 4 Nuclear contraction during wound induced cell death The hypocotyl of an agarose imbedded nuclear marker line (N6) was wounded with a razor blade and them imaged at 2 minute intervals by collecting confocal Z-series. Propidium iodide was included in the imaging buffer to monitor plasma membrane integrity. Following wounding,, the nucleus on the left displays hypertrophy relative to the normal lentoid shape of unwounded cells (see the nuclei in cells on the right hand side in the movie). Over the course of the time series, the nucleus contracts concomitant with a decrease in GFP fluorescence, most evident in the time series between the 16 and 20 minute time points shown. This is followed later by intense staining of the contracted nucleus by propidium iodide. |
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Source | Cutler S, Somerville C (2005). "Imaging plant cell death: GFP-Nit1 aggregation marks an early step of wound and herbicide induced cell death". BMC Plant Biology. DOI:10.1186/1471-2229-5-4. PMID 15796778. PMC: 1087855. | ||
Author | Cutler S, Somerville C | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 19:48, 5 December 2012 | 3.3 s, 502 × 226 (192 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional File 4 |
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Author | Cutler S, Somerville C |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Nuclear contraction during wound induced cell death The hypocotyl of an agarose imbedded nuclear marker line (N6) was wounded with a razor blade and them imaged at 2 minute intervals by collecting confocal Z-series. Propidium iodide was included in the imaging buffer to monitor plasma membrane integrity. Following wounding,, the nucleus on the left displays hypertrophy relative to the normal lentoid shape of unwounded cells (see the nuclei in cells on the right hand side in the movie). Over the course of the time series, the nucleus contracts concomitant with a decrease in GFP fluorescence, most evident in the time series between the 16 and 20 minute time points shown. This is followed later by intense staining of the contracted nucleus by propidium iodide. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2005 |