File:Human-Eosinophils-Express-the-High-Affinity-IgE-Receptor-FcεRI-in-Bullous-Pemphigoid-pone.0107725.s002.ogv
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[edit]DescriptionHuman-Eosinophils-Express-the-High-Affinity-IgE-Receptor-FcεRI-in-Bullous-Pemphigoid-pone.0107725.s002.ogv |
English: Representative interaction of FcεRIα and FcεRIβ on the surface of eosinophils in BP lesions. Interaction of specific molecules was evaluated using the proximity ligation assay on non-permeabilized skin cryosections from BP patients. Eosinophils were identified by their unique bi-lobed nuclear morphology (DAPI stain, blue) on z-series image stacks using high resolution confocal microscopy. Interaction of FcεRIα/FcεRIβ on tissue eosinophils from BP patient results in a red fluorescent signal. Circled area contains several eosinophils. The image stacks have been smoothed, annotated and pseudo-colored in NIH ImageJ. Scale bar = 50 uM. |
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Source | Movie S2 from Messingham K, Holahan H, Frydman A, Fullenkamp C, Srikantha R, Fairley J (2014). "Human Eosinophils Express the High Affinity IgE Receptor, FcεRI, in Bullous Pemphigoid". PLOS ONE. DOI:10.1371/journal.pone.0107725. PMID 25255430. PMC: 4177878. | |||
Author | Messingham K, Holahan H, Frydman A, Fullenkamp C, Srikantha R, Fairley J | |||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:52, 10 December 2014 | 3.6 s, 2,048 × 2,048 (1.14 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Messingham K, Holahan H, Frydman A, Fullenkamp C, Srikantha R, Fairley J |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Representative interaction of FcεRIα and FcεRIβ on the surface of eosinophils in BP lesions. Interaction of specific molecules was evaluated using the proximity ligation assay on non-permeabilized skin cryosections from BP patients. Eosinophils were identified by their unique bi-lobed nuclear morphology (DAPI stain, blue) on z-series image stacks using high resolution confocal microscopy. Interaction of FcεRIα/FcεRIβ on tissue eosinophils from BP patient results in a red fluorescent signal. Circled area contains several eosinophils. The image stacks have been smoothed, annotated and pseudo-colored in NIH ImageJ. Scale bar |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |