File:HAM-5-Functions-As-a-MAP-Kinase-Scaffold-during-Cell-Fusion-in-Neurospora-crassa-pgen.1004783.s010.ogv
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[edit]DescriptionHAM-5-Functions-As-a-MAP-Kinase-Scaffold-during-Cell-Fusion-in-Neurospora-crassa-pgen.1004783.s010.ogv |
English: Oscillatory movement of HAM-5-GFP and SO-mCherry to opposite CAT tips in two germlings undergoing chemotropic interactions. A 52-minute movie compressed in 15 seconds of two genetically identical ham-5-gfp; so-mCherry germlings undergoing chemotropic interactions and cell fusion. Oscillatory recruitment of HAM-5-GFP and SO-mCherry to opposite CAT tips of the germlings is observed. Ham-5-GFP is also observed at the site of germling contact, while SO-mCherry is not. After fusion and cytoplasm mixing, the HAM-5-FGP and SO-mCherry fluorescent proteins are observed in both germlings. The upper left panel is made of brightfield images, the lower left of GFP-fluorescent images, the lower right of mCherry-fluorescent images and the upper right panel is a movie of merged GFP and mCherry fluorescent images. |
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Source | Movie S3 from Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N (2014). "HAM-5 Functions As a MAP Kinase Scaffold during Cell Fusion in Neurospora crassa". PLOS Genetics. DOI:10.1371/journal.pgen.1004783. PMID 25412208. PMC: 4238974. | |||
Author | Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N | |||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:46, 10 December 2014 | 15 s, 1,024 × 1,024 (3.32 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Jonkers W, Leeder A, Ansong C, Wang Y, Yang F, Starr T, Camp D, Smith R, Glass N |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Oscillatory movement of HAM-5-GFP and SO-mCherry to opposite CAT tips in two germlings undergoing chemotropic interactions. A 52-minute movie compressed in 15 seconds of two genetically identical ham-5-gfp; so-mCherry germlings undergoing chemotropic interactions and cell fusion. Oscillatory recruitment of HAM-5-GFP and SO-mCherry to opposite CAT tips of the germlings is observed. Ham-5-GFP is also observed at the site of germling contact, while SO-mCherry is not. After fusion and cytoplasm mixing, the HAM-5-FGP and SO-mCherry fluorescent proteins are observed in both germlings. The upper left panel is made of brightfield images, the lower left of GFP-fluorescent images, the lower right of mCherry-fluorescent images and the upper right panel is a movie of merged GFP and mCherry fluorescent images. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-11 |