File:Filamin-and-Phospholipase-C--Are-Required-for-Calcium-Signaling-in-the-Caenorhabditis-elegans-pgen.1003510.s014.ogv

English: lfe-2(sy326) increases calcium release in fln-1(tm545) animals. A representative fln-1(tm545); lfe-2(sy326) spermathecal transit imaged using xbIs1101[fln-1p::GCaMP]. Oocyte entry occurs from the right at 30 seconds. The sp-ut valve is not constricted and oocyte entry partially dilates the valve. Increased calcium signaling compared to fln-1(tm545) single mutant animals pushes the oocyte partially out of the spermatheca. The movie is shown twenty times faster than real-time with a lookup table to visualize low intensities (cool colors represent low intensity). Time is shown as minutes∶seconds and begins 30 seconds before oocyte entry. The entire region shown (100 µm×50 µm) was used for the mean fluorescence intensity measurements in Figure 7B.