File:Fast-Multiphase-Volume-Adaptation-to-Hyperosmotic-Shock-by-Escherichia-coli-pone.0035205.s003.ogv

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Fast-Multiphase-Volume-Adaptation-to-Hyperosmotic-Shock-by-Escherichia-coli-pone.0035205.s003.ogv (Ogg Theora video file, length 2 min 15 s, 40 × 68 pixels, 3 kbps, file size: 47 KB)

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Description
English: A sequence of raw fluorescent images of a single E.coli cell prior and during a hyperosmotic shock showing fast water efflux. The cell is transferred from isoosmotic buffer (10 mM Tris-HCl buffer supplemented with 150 mM sucrose) to isoosmotic buffer and 620 mM sucrose. Cytoplasmic volume was marked with the green protein EGFP and total cell volume by the red outer membrane dye FM4-64. Images were acquired at a frame every 1.6 seconds alternating between the cytoplasmic volume and total cell volume.
Date
Source Video S1 from Pilizota T, Shaevitz J (2012). "Fast, Multiphase Volume Adaptation to Hyperosmotic Shock by Escherichia coli". PLOS ONE. DOI:10.1371/journal.pone.0035205. PMID 22514721. PMC: 3325977.
Author Pilizota T, Shaevitz J
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current21:17, 30 October 20122 min 15 s, 40 × 68 (47 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 1 kbps Completed 12:25, 17 June 2024 3.0 s
WebM 360P 1 kbps Completed 09:38, 30 November 2023 1.0 s
QuickTime 144p (MJPEG) 7 kbps Completed 01:48, 11 October 2024 0.0 s

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