File:Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s007.ogv

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Experimental-and-Computational-Analysis-of-Polyglutamine-Mediated-Cytotoxicity-pcbi.1000944.s007.ogv (Ogg Theora video file, length 14 s, 694 × 520 pixels, 399 kbps, file size: 666 KB)

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English: Time-lapse imaging of U87MG cells expressing HttQ103YFP-pIRES-mRFPu treated with PI 24 hours post-transfection. Cells were visualized using filters that detect YFP or RFP. Fluorescence emanating from the YFP and RFP channels was merged to create a movie which shows an increase of IB formation and UPS dysfunction. Images acquired every 10 minutes using a 10× objective for a total of 24 hours. The movie shows rapid and progressive accumulation of the mRFPu reporter protein (red colour) after the addition of PI. This coincides with the formation of IB at an earlier time point (30 hours). At the conclusion of the movie there are considerably more IB and cell death events as compared to untreated cells (videos S1 and S2).
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Source Video S4 from Tang M, Proctor C, Woulfe J, Gray D (2010). "Experimental and Computational Analysis of Polyglutamine-Mediated Cytotoxicity". PLOS Computational Biology. DOI:10.1371/journal.pcbi.1000944. PMID 20885783. PMC: 2944785.
Author Tang M, Proctor C, Woulfe J, Gray D
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current21:55, 17 November 201214 s, 694 × 520 (666 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 138 kbps Completed 07:16, 26 August 2018 6.0 s
VP9 360P 80 kbps Completed 07:16, 26 August 2018 4.0 s
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WebM 360P 446 kbps Completed 18:49, 19 November 2012 6.0 s
QuickTime 144p (MJPEG) 363 kbps Completed 04:39, 25 October 2024 1.0 s

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