File:Embryogenesis-of-the-First-Circulating-Endothelial-Cells-pone.0060841.s004.ogv

English: A wide-field recording, and two ROI panels at higher resolution, depicting events in a HH10 to HH18 Tie1-H2B-YFP embryo (see Fig. 3). The optical data represent three orders of spatial magnitude (µm-mm). The white boxes correspond to a ROI depicted in the panel to the right at higher magnification. The boxed area shows morphogenesis of the right (anatomical) vitelline artery. Single cells (nuclei) and clusters of Tie1-YFP cells can be seen moving with a wide variety of speeds compared to ‘stationary’ endothelial tubes (encircled nuclei, 0.0–3.7 h, 5.5–6.6 h, 6.8–7.7 h). Note the large aggregates of fluorescent cells in the tail bud region, which dissipate as the recording progresses (10.0 h large circle, left panel). As 17.0 h approaches hundreds of Tie1-YFP cells and cellular aggregates are observed flowing from caudal positions toward the nascent inflow tract of the heart as indicated by the white chevron. These empirical data show multiple examples of rapid displacement behavior. The recordings strongly suggest that large numbers of Tie1-YFP cellular aggregates shear off from the tail bud and enter systemic circulation. The recording rate is 9.1fph or 6.6 min between frames. The compression algorithms used in creating this movie result in a loss of resolution compared to the native image files (see Methods). Mag bars  = 100 µm.