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English: Oryctes rhinoceros nudivirus (OrNV) Virion trafficking inside the host cell. (A) Micrograph of an OrNV-infected cell showing virions encapsulated into a vesicle inside the nucleus. Scale bar, 1 μm. (A1) A 11-nm slice through the tomographic reconstruction indicated in inset showing virions enclosed in a double membrane vesicle. Scale bar, 500 nm. (A2) Segmentation of the tomogram. (B) Micrograph displaying expanded NE. Scale bar, 1 μm. (B1) A 11-nm slice through the tomographic reconstruction indicated in inset shows virions inside an expanded NE lumen. Scale bar, 500 nm. (B2) Segmentation of B1 highlights vesicle wrapped virions inside the NE lumen. (C) Micrograph shows cytoplasm completely traversing the nucleus. Scale bar, 1 μm. (C1) A 11-nm slice through the tomographic reconstruction of the area indicated in inset displaying virions encapsulated into multimembrane vesicles (MMVs) in the cytoplasm. Scale bar, 500 nm. (C2) Segmentation showing virions inside complex MMVs, and vesicle-free virions close to the cellular membrane. (D) An infected cell showing vesicles being expelled into the extracellular space. Scale bar, 1 μm. (D1) A slice through the tomographic reconstruction of the area indicated in inset showing vesicles containing fully enveloped virions. Scale bar, 500 nm. (D2) Segmentation showing virions within vesicles. |
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Fig. 3 at https://mbio.asm.org/content/11/4/e01333-20/figures-only Visualizing Nudivirus Assembly and Egress. In: ASM mBio 11:e01333-20. PMID 32788378; doi:10.1128/mBio.01333-20  |
| Author | Sailakshmi Velamoor, Allan Mitchell, Bruno M. Humbel, WonMo Kim, Charlotte Pushparajan, Gabriel Visnovsky, Laura N. Burga, Mihnea Bostina; Anne Moscona (Editor) |
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