File:Coupled-Protein-Diffusion-and-Folding-in-the-Cell-pone.0113040.s002.ogv
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Coupled-Protein-Diffusion-and-Folding-in-the-Cell-pone.0113040.s002.ogv (Ogg Theora video file, length 4.0 s, 103 × 124 pixels, 37 kbps, file size: 18 KB)
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[edit]DescriptionCoupled-Protein-Diffusion-and-Folding-in-the-Cell-pone.0113040.s002.ogv |
English: GFP bleaching. The movie of GFP photobleaching in the cell by FLIP discussed in the main text. The movie is generated by taking the snapshots generated every 10 s during the FLIP measurement and speeding the playback rate to 5 frames per second. The cell shape is selected by thresholding and pixels below the threshold (outside of the cell) are set to 0 for all snapshots. As described in the text, the nucleus of the cell is also selected and pixels inside the nucleus are excluded from simulation and set to 0 for the purpose of illustration. The raw data is obtained as a greyscale image and displayed green since all measured fluorescence is from the GFP. No other modifications or corrections are applied to the data. |
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Source | Movie S1 from Guo M, Gelman H, Gruebele M (2014). "Coupled Protein Diffusion and Folding in the Cell". PLOS ONE. DOI:10.1371/journal.pone.0113040. PMID 25436502. PMC: 4249841. | ||
Author | Guo M, Gelman H, Gruebele M | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 07:48, 25 March 2017 | 4.0 s, 103 × 124 (18 KB) | Revent (talk | contribs) | attempt to fix transcodes | |
04:39, 9 December 2014 | 4.0 s, 103 × 124 (10 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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