File:Cortactin-as-a-Target-for-FAK-in-the-Regulation-of-Focal-Adhesion-Dynamics-pone.0044041.s002.ogv

English: FA dynamics in GFP-FAK WT expressing cells. Cells were plated at subconfluent density on FN coated (2 µg/ml) glass bottom dishes (MatTek), serum starved (12 h), treated with growth media supplemented with 50 ng/ml EGF and imaged every 2 min for 1 h. Images were acquired every 2 min for 1 h in a humidified 5% CO2 environment at 37°C using an Olympus IX81 spinning disk confocal microscope, XY-controlled stage with zero drift compensation focus control at 60X (PlanApo, N.A. 1.42), using a mercury lamp source, multiband dichroic, single-band exciter, and single band emitter filter sets (Chroma) on dual filter wheels, and an OrcaER camera (Hamamatsu) controlled by Slidebook (v5.0) software. Videos were created and compressed using QuickTime Pro (Apple). 30 sequential images are shown at 10 frames per second.