File:Chronic-Parasitic-Infection-Maintains-High-Frequencies-of-Short-Lived-Ly6C+CD4+-Effector-T-Cells-ppat.1004538.s009.ogv
Chronic-Parasitic-Infection-Maintains-High-Frequencies-of-Short-Lived-Ly6C+CD4+-Effector-T-Cells-ppat.1004538.s009.ogv (Ogg Theora video file, length 5.9 s, 595 × 596 pixels, 543 kbps, file size: 391 KB)
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[edit]DescriptionChronic-Parasitic-Infection-Maintains-High-Frequencies-of-Short-Lived-Ly6C+CD4+-Effector-T-Cells-ppat.1004538.s009.ogv |
English: Dynamic imaging of tissue resident cells derived from adoptively transferred CD4+CD44+CD62L+GFP+ TCM cells following challenge with L. major-RFP. GFP-expressing cells are shown in green, the 2-photon second harmonic signal is shown in blue, and L. major-RFP is shown in red. Playback speed is 600×. Scale bar, 50 µm. Two photon intravital imaging and image analysis was performed as described previously [11], [38]. Raw imaging data were processed with Imaris (Biplane) using a Gaussian filter for noise reduction. All images are displayed as 2D maximum intensity projections. Movie files of 3-dimentional images were generated using Imaris. |
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Source | Movie S1 from Peters N, Pagán A, Lawyer P, Hand T, Henrique Roma E, Stamper L, Romano A, Sacks D (2014). "Chronic Parasitic Infection Maintains High Frequencies of Short-Lived Ly6C+CD4+ Effector T Cells That Are Required for Protection against Re-infection". PLOS Pathogens. DOI:10.1371/journal.ppat.1004538. PMID 25473946. PMC: 4256462. | |||
Author | Peters N, Pagán A, Lawyer P, Hand T, Henrique Roma E, Stamper L, Romano A, Sacks D | |||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:38, 13 December 2014 | 5.9 s, 595 × 596 (391 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Peters N, Pagán A, Lawyer P, Hand T, Henrique Roma E, Stamper L, Romano A, Sacks D |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
Image title | Dynamic imaging of tissue resident cells derived from adoptively transferred CD4+CD44+CD62L+GFP+ TCM cells following challenge with L. major-RFP. GFP-expressing cells are shown in green, the 2-photon second harmonic signal is shown in blue, and L. major-RFP is shown in red. Playback speed is 600×. Scale bar, 50 µm. Two photon intravital imaging and image analysis was performed as described previously [11], [38]. Raw imaging data were processed with Imaris (Biplane) using a Gaussian filter for noise reduction. All images are displayed as 2D maximum intensity projections. Movie files of 3-dimentional images were generated using Imaris. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014-12 |