File:Centrosome migration in a living Drosophila female interphase germline stem cell - pbio.1001357.s006.ogv
Centrosome_migration_in_a_living_Drosophila_female_interphase_germline_stem_cell_-_pbio.1001357.s006.ogv (Ogg Theora video file, length 4.3 s, 512 × 512 pixels, 73 kbps, file size: 38 KB)
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[edit]DescriptionCentrosome migration in a living Drosophila female interphase germline stem cell - pbio.1001357.s006.ogv |
English: Centrosome migration in a living female interphase GSC. In this movie, one GSC centrosome is localized at the niche-GSC interface, while the other centrosome moves between 10° and 15° around the circumference of the GSC toward the posterior. Figure S2 contains representative frames from this movie. In this movie and in Movie S2, centrosomes are marked by a GFP-tagged pericentrosomal protein Centrosomin (GFP-Cnn, shown in green) under the control of germline-specific Nos-Gal4 driver. The nuclei of the somatic niche cells (and to a lesser extent, the undifferentiated anterior germline cells) are marked by a living-cell DNA dye (Draq5-Cy5, shown in red). The boundary of Cnn-GFP and bright Draq5 staining delineates the CpC-GSC interface. Each movie covers about 30 min of development, with one frame taken every minute. Each frame is a projection of a stack of eight 1 µm confocal slices. The images were taken using a 63× water lens of a DSU Olympus Spinning Disk Confocal Microscope (see more details in Text S1 ).
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Source | Movie S1 from Lu W, Casanueva M, Mahowald A, Kato M, Lauterbach D, Ferguson E, Montell D. "Niche-Associated Activation of Rac Promotes the Asymmetric Division of Drosophila Female Germline Stem Cells". PLoS Biology. DOI:10.1371/journal.pbio.1001357. PMID 22802725. PMC: 3389017. | ||
Author | Lu W, Casanueva M, Mahowald A, Kato M, Lauterbach D, Ferguson E, Montell D | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 16:15, 24 August 2012 | 4.3 s, 512 × 512 (38 KB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Movie S1 |
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Author | Lu W, Casanueva M, Mahowald A, Kato M, Lauterbach D, Ferguson E, Montell D |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-07-03 |